In this research, a series of experimental and numerical studies on the thermal performance of a single-turn PULSATING Heat Pipe (PHP) using distilled water as base FLUID and distilled water including micro-coppers were performed. Thermal resistance, average temperatures of the hot region (evaporator), average temperatures of the cold part (condenser), and two-phase flow regime of the system were investigated at the different filling ratios, at input powers (20, 30, 40, 50 and 60W), at a concentration of micro-coppers (0. 0625 g/mL). The oscillating heat pipe was fabricated with a copper capillary tube by choosing the internal and external diameters of 4 mm and 6 mm, respectively. Experiments showed adding micro-coppers into base FLUID improves the main mechanism of the PHP based on the oscillating motion of vapor plugs and liquid slugs. The lowest thermal resistance of the system at a filling ratio of 40%, at a concentration of micro-coppers (0. 0625 g/ml), at heat input (60 W) was 0. 95 deg C/W. Meanwhile, CFD results illustrated adding micro-coppers into base FLUID increases the turbulence intensity of the system especially in the evaporator up to 45% which enhances the heat transfer through the PHP in comparison to base FLUID.